Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1

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Cat. No. / Size
Price
Qty
IGG-BLS307-25ug
$470.00
IGG-BLS307-200ug
$2035.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a mouse monoclonal antibody recombinantly expressed in CHO cells.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Conjugate

    Biotin

  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     IgG Fc ELISA

    Immobilized Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) with a linear range of 0.03-4 ng/mL (QC tested).

    Protocol
  •  IgG Fc ELISA

    Binding activity of Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) to different Human IgGs, as determined by ELISA. Human IgGs were coated at 1 μg/mL. The primary antibody (Cat. No. IGG-BLS307) was serially diluted starting from 62.5 ng/mL, and Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (Acro, Cat. No. STN-NH913) was used as the secondary antibody at a dilution of 0.1 μg/mL. Binding was measured by OD₄₅₀ (Routinely tested).

    Protocol

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Background

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).

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