Product Details
Source
Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a mouse monoclonal antibody recombinantly expressed in CHO cells.
Antibody Type
Recombinant Monoclonal
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Conjugate
Biotin
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) with a linear range of 0.03-4 ng/mL (QC tested).
Protocol
Binding activity of Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) to different Human IgGs, as determined by ELISA. Human IgGs were coated at 1 μg/mL. The primary antibody (Cat. No. IGG-BLS307) was serially diluted starting from 62.5 ng/mL, and Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (Acro, Cat. No. STN-NH913) was used as the secondary antibody at a dilution of 0.1 μg/mL. Binding was measured by OD₄₅₀ (Routinely tested).
Protocol
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