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Product Details
Source
Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a recombinant mouse monoclonal antibody expressed in CHO cells.
Antibody Type
Recombinant Monoclonal
Clone
6F11C8
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Human IgG Fc, including IgG1–IgG4 isotypes and Fc-engineered variants (e.g. N297A, LALA, YTE, LS, S239D/I332E)
Immunogen
Recombinant Human Fc derived from CHO cells
Specificity
Specifically binds to the Fc region of human IgG without recognizing Fab fragments or mouse IgG isotypes.
Application
ApplicationRecommended UsageELISA1:15000-1:150000Cross Verification
Human Fc recognition This antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, IgG4) and multiple Fc-engineered variants, including N297A, LALA, YTE, LS, S239D/I332E, etc.
Species cross-reactivity
• Weak cross-reactivity with cynomolgus IgG was observed by ELISA.
• No detectable binding to mouse IgG isotypes (IgG1, IgG2a, IgG2b).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) at 2μg/mL (100μL/well) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8)(Cat. No. IGG-S307) with a linear range of 0.05-3.13 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (MALS verified) (Cat. No. SPD-M400a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (MALS verified) (Cat. No. SPD-M401a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) and Human IgG1 Fc (MALS verified) (Cat. No. AC2-H5257) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8) (Cat. No. IGG-S307). The antibody does not bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (MALS verified) (Cat. No. S1N-M164) and Human CD19 (20-291) Protein, His Tag DMF Filed (Cat. No. CD9-H52H2) (Routinely tested).
Protocol
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