製品詳細
Product Overview
rHuman Activin A ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate recombinant human Activin A that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteActivin AFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity20 pg/mLRange31.25 pg/mL-2000 pg/mLAssay Time2.75 hrSample volume50 μLSample TypeFor the quantitative determination of rHuman Activin A in BSS.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of recombinant human Activin A in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA208-C01Pre-coated Anti-Activin A Antibody Microplate1 plateCEA208-C02rHuman Activin A Standard20 ng×2CEA208-C03Biotin-Anti-Activin A Antibody Con. Solution100 μLCEA208-C04Biotin-Antibody Dilution Buffer8 mLCEA208-C05Streptavidin-HRP Con. Solution500 μLCEA208-C06Streptavidin-HRP Dilution Buffer15 mLCEA208-C0720× Washing Buffer50 mLCEA208-C08Sample Dilution Buffer15 mL×2CEA208-C09Substrate Solution12 mLCEA208-C10Stop Solution6 mLACRO Quality Management System
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データ表示
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of recombinant Activin A is less than 20 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different Activin A concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of Activin A were tested in independent assays. Acceptable criteria: CV<15%.

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