Human Amyloid beta 1-40 ELISA Kit

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製品番号/仕様
価格
数量
CEA-B248-96tests
$565.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Product Overview

    Human Amyloid beta 1-40 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant humanAmyloid beta 1-40 that is present in biological samples. Our ELISA Kit provides several benefits:

    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    Amyloid beta 1-40
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    400 pg/mL
    Assay Time
    2.25 hr
    Sample volume
    20 μL
    Range
    447 pg/mL-7500 pg/mL
    Sample Type
    For the quantitative determination of human Amyloid beta 1-40 in Cerebrospinal Fluid.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human Amyloid beta 1-40 in cerebrospinal fluid.

    It is for research use only.

  • Workflow

    Amyloid beta 1-40 Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA248-C01
    Pre-coated Anti-Amyloid beta 1-40 Antibody Microplate
    1 plate
    CEA248-C02
    Human Amyloid beta 1-40 Standard
    3 μg×2
    CEA248-C03
    Biotin-Anti-Amyloid beta 1-40 Antibody Con. Solution
    100 μL
    CEA248-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA248-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA248-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA248-C07
    20×Washing Buffer
    50 mL
    CEA248-C08
    Sample Dilution Buffer
    15 mL×2
    CEA248-C09
    Substrate Solution
    12 mL
    CEA248-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Typical Data 

    Please refer to DS document for the assay protocol.

     Amyloid beta 1-40 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Amyloid beta 1-40 is less than 400 pg/mL.

Validation
  • Sample Values

    Ten healthy Cerebrospinal Fluid samples were evaluated for the concentrations of human Amyloid beta 1-40 in assay.

     Amyloid beta 1-40 SAMPLE VALUES
  • Intra-Assay Statistics

    Ten replicates of each of three samples containing different Amyloid beta 1-40 concentrations were tested in one assay. Acceptable criteria: CV<10%.

     Amyloid beta 1-40 INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Three samples containing different concentrations of Amyloid beta 1-40 were tested in independent assays. Acceptable criteria: CV<15%.

     Amyloid beta 1-40 INTER-ASSAY STATISTICS
  • Recovery

    Recombinant Amyloid beta 1-40 was spiked into three human Cerebrospinal Fluid samples, and then analyzed. The average recovery of Amyloid beta 1-40 for Cerebrospinal Fluid samples is 93.7%.

     Amyloid beta 1-40 RECOVERY

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