Human CCL5/RANTES ELISA Kit

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製品番号/仕様
価格
数量
CEA-C088-96tests
$515.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Product Overview

    Human CCL5/RANTES ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human CCL5/RANTES that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    CCL5/RANTES
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    15.63 pg/mL
    Assay Time
    1.75 hr
    Sample volume
    50 μL
    Range
    15.63 pg/mL-1000 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    92/520

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human CCL5/RANTES in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    CCL5 Workflow

  • Storage

    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CEA088-C01
    Pre-coated Anti-CCL5 Antibody Microplate
    1 plate
    CEA088-C02
    Human CCL5 Standard
    500 μL
    CEA088-C03
    Biotin-Anti-CCL5 Antibody Con. Solution
    100 μL
    CEA088-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CEA088-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CEA088-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CEA088-C07
    20×Washing Buffer
    50 mL
    CEA088-C08
    2×Sample Dilution Buffer
    15 mL×2
    CEA088-C09
    Substrate Solution
    12 mL
    CEA088-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Typical Data 

    Please refer to DS document for the assay protocol.

     CCL5 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL5/RANTES is less than 15.63 pg/mL.

Validation
  • Sample Values

    Serum Sample: Ten healthy serum samples were evaluated for the concentrations of human CCL5/RANTES in assay.

     CCL5 SAMPLE VALUES
  • Intra-Assay Statistics

    Ten replicates of each of four samples containing different CCL5/RANTES concentrations were tested in one assay. Acceptable criteria: CV<10%.

     CCL5 INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five samples containing different concentrations of CCL5/RANTES were tested in independent assays. Acceptable criteria: CV<15%.

     CCL5 INTER-ASSAY STATISTICS
  • Recovery

    Recombinant CCL5/RANTES was spiked into three human serum samples, and then analyzed. The average recovery of CCL5/RANTES for serum samples is 92.5%.

     CCL5 RECOVERY

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医薬品開発の進捗
  • 英語名:

    C-C motif chemokine 5

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