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Product Details
Product Overview
Human CXCL9/MIG ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human CXCL9/MIG that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteCXCL9/MIGFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity20 pg/mLAssay Time2.75 hrSample volume50 μLRange31.25 pg/mL-2000 pg/mLSample TypeFor the quantitative determination of human CXCL9/MIG in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human CXCL9/MIG in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA220-C01Pre-coated Anti-CXCL9/MIG Antibody Microplate1 plateCEA220-C02Human CXCL9/MIG Standard5 μg/mL×2CEA220-C03Biotin-Anti-CXCL9/MIG Antibody Con. Solution100 μLCEA220-C04Biotin-Antibody Dilution Buffer8 mLCEA220-C05Streptavidin-HRP Con. Solution500 μLCEA220-C06Streptavidin-HRP Dilution Buffer15 mLCEA220-C0720× Washing Buffer50 mLCEA220-C08Sample Dilution Buffer15 mL×2CEA220-C09Substrate Solution12 mLCEA220-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CXCL9/MIG is less than 20 pg/mL.
Sample Values
Twenty-four healthy serum samples were evaluated for the concentrations of human CXCL9/MIG in assay. The detection human CXCL9/MIG rate of the samples was 100%, the range was 74.905 - 868.877 pg/mL, and the mean detectable value was 256.794 pg/mL.
Dilution Linearity
Three Serum samples with high concentrations of native CXCL9/MIG were serially diluted with dilution buffer to produce samples with values within the dynamic range of the assay and then assayed. The average recovery of CXCL9/MIG for serum samples is 109.33%.
Intra-Assay Statistics
Eight replicates of each of 3 samples containing different CXCL9/MIG concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of CXCL9/MIG were tested in independent assays. Acceptable criteria: CV<15%.
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