Human HGF ELISA Kit

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製品番号/仕様
価格
数量
CRS-B012-96tests
$565.00
CRS-B012-480tests (96tests X 5)
$2545.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Product Overview

    Human HGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human HGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    HGF
    Format
    96-wells plate breakable into 12 x 8 wells strips
    Reactivity
    Human
    Sensitivity
    40 pg/mL
    Assay Time
    1 hr 45 min
    Sample volume
    50 μL
    Range
    62.5 pg/mL-8000 pg/mL
    Sample Type
    Cell Culture Supernatants, Plasma, Serum.
    NIBSC Code
    96/564

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Application

    The kit is developed for quantitative detection of natural and recombinant human HGF in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow

    HGF Workflow

  • Storage

    Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    CRB012-C01
    Pre-coated Anti-HGF Antibody Microplate
    1 plate
    CRB012-C02
    Human HGF Standard
    20 μg×2
    CRB012-C03
    Biotin-Anti-HGF Antibody Con. Solution
    100 μL
    CRB012-C04
    Biotin-Antibody Dilution Buffer
    8 mL
    CRB012-C05
    Streptavidin-HRP Con. Solution
    500 μL
    CRB012-C06
    Streptavidin-HRP Dilution Buffer
    15 mL
    CRB012-C07
    20×Washing Buffer
    50 mL
    CRB012-C08
    1×Dilution Buffer
    15 mL×2
    CRB012-C09
    Substrate Solution
    12 mL
    CRB012-C10
    Stop Solution
    6 mL
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Typical Data 

    Please refer to DS document for the assay protocol.

     HGF TYPICAL DATA

    For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.

Validation
  • Sample Values

    Serum Sample: Twenty healthy serum samples were evaluated for the concentrations of human HGF in assay.

     HGF SAMPLE VALUES

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    Hepatocyte growth factor

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