Human IFN-gamma Detection Kit (TR-FRET)

製品番号/仕様
価格
数量
FRT-C01-100tests
$450.00
FRT-C01-500tests
$780.00
FRT-C01-5000tests (500tests X 10)
$5200.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • FAB

    1. Comprehensive validation - Rigorously validated through comprehensive methodology to ensure reliable and accurate results.
    2. Simple and fast operation - No complicated washing steps, significantly reducing time.
    3. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
    4. Accurate and reliable results - High sensitivity with minimal matrix effects.
    5. High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
    6. Fast completion - Results in just 2 hours.
  • Product Overview

    Human IFN-gamma Detection kit (TR-FRET) can detect the human IFN-gamma in homogeneous system within 2 hours, it is highly sensitive, short detection time, easy to use and possesses high-throughput screening capability. Compatible with microplate formats, this kit supports high-throughput screening workflows, enabling efficient large-scale sample analysis. This kit is specifically designed for the accurate quantitation of human IFN-gamma from cell culture supernatant, serum and plasma.

  • Product Specifications

    Assay Type
    Sandwich-TR-FRET
    Analyte
    Human IFN-gamma
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    LOQ=46.64 pg/mL
    Standard Curve Range
    46.64 pg/mL-4000 pg/mL
    Assay Time
    2 hr
    Suitable Sample Type
    For human Human IFN-gamma detection in human serum, human plasma,cell culture supernatants
    Sample volume
    16 μL

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Workflow

    IFN-γ Workflow

  • Storage

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    FRTC01-C01
    Anti-Human IFN-γ Antibody Europium-chelate
    100 tests/500 tests
    FRTC01-C02
    FA Labeled Anti-Human IFN-γ Antibody
    100 tests/500 tests
    FRTC01-C03
    Human IFN-γ Standard
    20 μg/100 tests & 500 tests
    DB-04
    TR-FRET Sample Dilution Buffer, pH7.4
    50 mL/100 tests & 500 tests
    DB-05
    TR-FRET Detection Buffer, pH7.4
    50 mL/100 tests & 500 tests
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Typical Data 

    Please refer to DS document for the assay protocol.

     IFN-γ TYPICAL DATA

    Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04).

Validation
  • Dilution Linearity

    To evaluate the linearity of the assay, samples spiked with high-concentration human IFN-gamma in various matrices were prepared. With DB-04 (TR-FRET Sample Dilution Buffer, pH 7.4) as the surrogate matrix, standard curves were generated and the spiked samples were subjected to serial gradient dilution, followed by the quantification of the spiked samples.

     IFN-γ DILUTION LINEARITY
  • Intra-Assay Statistics

    Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy.

     IFN-γ INTRA-ASSAY STATISTICS
  • Inter-Assay Statistics

    Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.

     IFN-γ INTER-ASSAY STATISTICS

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