製品詳細
Product Overview
Human IL-12p70 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-12p70 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-12p70Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.0 pg/mLAssay Time1.75 hrSample volume50 μLRange3.90 pg/mL-500 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code95/544Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-12p70 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow

Storage
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRB009-C01Pre-coated Anti-IL-12 Antibody Microplate1 plateCRB009-C02Human IL-12p70 Standard20 μg×2CRB009-C03Biotin-Anti-IL-12 Antibody Con. Solution100 μLCRB009-C04Biotin-Antibody Dilution Buffer8 mLCRB009-C05Streptavidin-HRP Con. Solution500 μLCRB009-C06Streptavidin-HRP Dilution Buffer15 mLCRB009-C0720×Washing Buffer50 mLCRB009-C081×Dilution Buffer15 mL×2CRB009-C09Substrate Solution12 mLCRB009-C10Stop Solution6 mLACRO Quality Management System
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データ表示
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B009 is less than 2.0 pg/mL.
Sample Values
Serum Sample: Two hundred and forty healthy serum samples were evaluated for the concentrations of human IL-12p70 in assay.

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