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製品詳細
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-2Format96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity< 39.06 pg/mLStandard Curve Range39.06 pg/mL-2500 pg/mLAssay Time2 hr 50 minSuitable Sample TypeFor the quantitative determination of human IL-2 in Cell Culture Supernatants, Plasma, Serum.Sample volume100 μLProduct Overview
Human Interleukin-2 (IL-2) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-2 in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-2 (ACROBiosystems, cat#GMP-L02H14) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human IL-2.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCRS003-C01Pre-coated Anti-IL-2 Antibody Microplate1 plate (8×12 strips)CRS003-C02Human IL-2 Standard20 μgCRS003-C03Biotin-Anti-IL-2 Antibody50 μLCRS003-C04Streptavidin-HRP50 μLCRS003-C0510xWashing Buffer50 mLCRS003-C062xDilution Buffer50 mLCRS003-C07Substrate Solution12 mLCRS003-C08Stop Solution7 mLACRO Quality Management System
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データ表示
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IL-2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Intra-Assay Statistics
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
Recovery
Three parts of blank serum were added with different concentrations of human IL-2, and the serum without human IL-2 was used as background to calculate the recovery rate. The range of the recovery rate is 83.9-98.2%, and the average recovery is 90.2%.
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最新の進展
医薬品開発の進捗- 英語名：
Interleukin-2
- カテゴリ：
- 市販の医薬品数：
2 詳細
- 治験医薬品数：
46 詳細
- 最新の開発ステージ：
Approved
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