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製品詳細
Product Overview
Human Lipocalin-2/NGAL ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Lipocalin-2/NGAL that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteLipocalin-2/NGALFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity0.156 ng/mLAssay Time2.75 hrSample volume50 μLRange0.156 ng/mL-10 ng/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human Lipocalin-2/NGAL in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA204-C01Pre-coated Anti-NGAL Antibody Microplate1 plateCEA204-C02Human NGAL Standard20 μg×2CEA204-C03Biotin-Anti-NGAL Antibody Con. Solution100 μLCEA204-C04Biotin-Antibody Dilution Buffer8 mLCEA204-C05Streptavidin-HRP Con. Solution500 μLCEA204-C06Streptavidin-HRP Dilution Buffer15 mLCEA204-C0720×Washing Buffer50 mLCEA204-C082×Sample Dilution Buffer15 mL×2CEA204-C09Substrate Solution12 mLCEA204-C10Stop Solution6 mLACRO Quality Management System
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データ表示
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of NGAL is less than 0.156 ng/mL.
Sample Values
Serum Sample: NGAL concentrations were measured in eight human serum samples, which were collected from healthy human subjects. The measurements of eight samples are shown in the figure below.
Dilution Linearity
High concentrations of human NGAL serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of NGAL was 102.3%.
Intra-Assay Statistics
Ten replicates of each of four samples containing different NGAL concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Four samples containing different concentrations of NGAL were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
NGAL was spiked into three human serum samples, and then analyzed. The average recovery of NGAL for serum samples is 89.5%.
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