Human 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag, active trimer, low endotoxin (MALS verified)

Synonyms

4-1BB Ligand, TNFSF9, CD137L

Source

Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (41L-H52D4) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).

Predicted N-terminus: His

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process