Human 5-HT2AR Protein (S162K, M164W, S372N), Flag,His Tag (Nanodisc-pro)

Application

1. Cell based assay (FACS or functional assay)
2. Affinity testing (SPR / BLI)
3. Screening (ELISA)

Synonyms

5-HT2A, HTR2A, HTR2, 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A

Source

Nanodisc-pro Human 5-HT2AR Protein (S162K, M164W, S372N), Flag,His Tag (5HR-H55D4) is expressed from Baculovirus-Insect cells. It contains AA Asp 2- Ala 265 & Gln 313 - Lys 403 (Accession # P28223-1 (S162K, M164W, S372N)).

Predicted N-terminus: Asp

Molecular Characterization

This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 55.3 kDa.

*The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.

1. Structure

   

   Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".

2. Synthesis Process

   

   Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process