Monkeypox virus (Democratic Republic of the Congo) A33R Protein, Twin-Strep Tag&His Tag

Synonyms

A33R (MPXV)

Source

Monkeypox virus (Democratic Republic of the Congo) A33R Protein, Twin-Strep Tag&His Tag (A3R-M5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asp 142 (Accession # EPI_ISL_19093812, GISAID).

Predicted N-terminus: Trp

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a Strep Tag and HSA tag at the N-terminus and a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 88.7 kDa. The protein migrates as 95-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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