Human ACHE / Acetylcholinesterase Protein, His Tag (active enzyme)

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製品番号/仕様
価格
数量
AHE-H52H3-20ug
$390.00
AHE-H52H3-500ug
$3525.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    acetylcholinesterase (Yt blood group), Acetylcholinesterase, ACHE, apoptosis-related acetylcholinesterase, ARACHE, EC 3.1.1, EC 3.1.1.7, N-ACHE, Yt blood group, YT

  • Source

    Human ACHE, His Tag (AHE-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 32 - Leu 614 (Accession # P22303-1).

    Predicted N-terminus: Glu 32

    Request for sequence
  • Molecular Characterization

    ACHE Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 66.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    ACHE SDS-PAGE

    Human ACHE, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity

    Measured by its ability to cleave Acetylthiocholine. The specific activity is >500 nmol/min/μg, as measured under the described conditions (QC tested).

    Protocol

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バックグランド

The enzyme acetylcholinesterase, also known as AChE or acetylhydrolase, is the primary cholinesterase in the body. It is a serine hydrolase whose primary function is to catalyzes the breakdown of acetylcholine(ACh) and of some other choline esters that function as neurotransmitters. AChE is found at mainly neuromuscular junctions and in chemical synapses of the cholinergic type, where its activity serves to terminate synaptic transmission. Acetylcholine deficit has been found in Alzheimer's disease (AD) ans is associated with cognetive decline. Therefore, AChE has been reported to be a viable therapeutic target for the treatment of AD and other dementias. To this end, acetylcholinesterase inhibitors (AChEIs) are commonly used.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Acetylcholinesterase

  • カテゴリ:

  • 市販の医薬品数:

    27 詳細

  • 治験医薬品数:

    36 詳細

  • 最新の開発ステージ:

    Approved

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