Human B7-H5 / Gi24 / VISTA Protein, Mouse IgG2a Fc Tag, low endotoxin

ユーザーレビュー
製品番号/仕様
価格
数量
B75-H5258-100ug
$310.00
B75-H5258-1mg (500ug X 2)
$2245.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    B7-H5, SISP1, Gi24, VISTA

  • Source

    Human B7-H5 Protein, Mouse IgG2a Fc Tag (B75-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1).

    Predicted N-terminus: Phe 33

    Request for sequence
  • Molecular Characterization

    B7-H5 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

お客様はこちらも閲覧

データ表示

  • SDS-PAGE

    B7-H5 SDS-PAGE

    Human B7-H5 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     B7-H5 ELISA

    Immobilized Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human B7-H5 Protein, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) with a linear range of 0.039-0.625 μg/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     B7-H5 SPR

    Human B7-H5 Protein, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag (Cat. No. VS3-H5258) with an affinity constant of 8.87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

    Protocol
  • Bioactivity-BLI

     B7-H5 BLI

    Loaded Biotinylated Human VSIG3, Fc,Avitag (Cat. No. VS3-H82F9) on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag (Cat. No. B75-H5258) with an affinity constant of 2.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

ユーザーレビュー
コメントを投稿

バックグランド

Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    V-domain Ig suppressor of T-cell activation

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    8 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

お問い合わせ
医薬品候補ライセンス
他の関連情報
  • 製品情報
  • 製品詳細
  • お客様はこちらも閲覧
  • データ表示
  • ユーザーレビュー
  • 背景
お問い合わせ

お問い合わせ
グローバル:+1 800-810-0816(Toll Free)

メールアドレス
order.jp@acrobiosystems.com

アクロバイオシステムズ株式会社
〒101-0051
東京都千代田区神田神保町1-32-8