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製品詳細
Synonyms
B7-H7, HHLA2, B7 Homolog 7
Source
Human B7-H7, His Tag (B77-H52H6) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human B7-H7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human B7-H7, His Tag (Cat. No. B77-H52H6) is more than 95% and the molecular weight of this protein is around 47-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Human B7-H7, His Tag (Cat. No. B77-H52H6) with a linear range of 0.078-2.5 μg/mL (QC tested).
Protocol
Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H6) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 10-156 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human KIR3DL3 / CD158z Protein, Fc,Avitag (Cat. No. KI3-H82F3) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human B7-H7, His Tag (Cat. No. B77-H52H6) with a linear range of 0.02-5 μg/mL (Routinely tested).
Protocol
ユーザーレビュー コメントを投稿
A***r2025-11-15
いいねI have been using ACRO Biosystems products since 2021, especially DLL4 Fc proteins (DL4-H5259), refer to our published article: https://pubmed.ncbi.nlm.nih.gov/37795096/, where we have used Acro-products and obtained excellent results. Recently, I ordered Human B7-H7 / HHLA2 Protein, His Tag (B77-H52H6-100 µg) for my ELISA assay, and it performed exceptionally well-as expected (Unpublished data). I am very satisfied with the consistent quality and reliability of ACRO’s products. Thanks, Ankit Tanwar Staff Scientist AECOM, NY
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最新の進展
医薬品開発の進捗- 英語名：
HERV-H LTR-associating protein 2
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
2 詳細
- 最新の開発ステージ：
Phase 1 Clinical
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