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Product Details
Synonyms
BAFFR, TNFRSF13C, BROMIX, CD268, CVID4, prolixin, BAFF-R
Source
Cynomolgus BAFFR Protein, Fc Tag (BAR-C5253) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Gly 76 (Accession # A0A2K5V1K1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 33.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus BAFFR Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus BAFFR Protein, Fc Tag (Cat. No. BAR-C5253) is more than 90% and the molecular weight of this protein is around 66-86 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus BAFFR Protein, Fc Tag (Cat. No. BAR-C5253) at 1 μg/mL (100 μL/well) can bind Human Anti-BAFFR Antibody, Human IgG1 | Kappa with a linear range of 0.03-8 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cynomolgus BAFFR Protein, Fc Tag (Cat. No. BAR-C5253) captured on Protein A Chip can bind Cynomolgus BAFF Protein, His Tag, active trimer (Cat. No. BAF-C5243) with an affinity constant of 2.14 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell activating factor receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
14 Details
- Highest Development Stage：
NDA/BLA
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