Human BCMA / TNFRSF17 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
BCA-H5253-100ug
$360.00
BCA-H5253-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    TNFRSF17, CD269, BCM, BCMA

  • Source

    Human BCMA, Mouse IgG2a Fc Tag (BCA-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    BCMA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 32.8 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    BCMA SDS-PAGE

    Human BCMA, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    BCMA SEC-MALS

    The purity of Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) is more than 95% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     BCMA ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.2-6 ng/mL (QC tested).

    Protocol
  •  BCMA ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA MAb with a linear range of 0.2-3 ng/mL (Routinely tested).

    Protocol
  •  BCMA ELISA

    Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).

    Protocol
  •  BCMA ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL, add increasing concentrations of Teclistamab and then add Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) at 1 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 20.12 ng/mL (Routinely tested).

    Protocol
  •  BCMA ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL, add increasing concentrations of Teclistamab and then add Biotinylated Human CD3 epsilon, His,Avitag (Cat. No. CDE-H82E1) at 0.2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 6.23 ng/mL (Routinely tested).

    Protocol
  •  BCMA ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL, add increasing concentrations of Teclistamab and then add HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) at 1 μg/mL, with sensitivity of 3.67 ng/mL (Routinely tested).

    Protocol

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バックグランド

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    B-cell maturation protein

  • カテゴリ:

  • 市販の医薬品数:

    8 詳細

  • 治験医薬品数:

    145 詳細

  • 最新の開発ステージ:

    Approved

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