Human BTLA (31-150) (M105V, G138S, V148M) Protein, Fc Tag (MALS verified)

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製品番号/仕様
価格
数量
BTA-H5254-100ug
$360.00
BTA-H5254-1mg
$2040.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    BTLA, CD272

  • Source

    Human BTLA (31-150) (M105V, G138S, V148M), Fc Tag (BTA-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1 (M105V, G138S, V148M)).

    Predicted N-terminus: Lys 31

    Request for sequence
  • Molecular Characterization

    BTLA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.2 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    BTLA SDS-PAGE

    Human BTLA (31-150) (M105V, G138S, V148M), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    BTLA SEC-MALS

    The purity of Human BTLA (31-150) (M105V, G138S, V148M), Fc Tag (Cat. No. BTA-H5254) is more than 85% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     BTLA ELISA

    Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150) (M105V, G138S, V148M), Fc Tag (Cat. No. BTA-H5254) with a linear range of 0.02-0.8 ng/mL (QC tested).

    Protocol

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よくある質問

  • Product

    Why are different expression ranges (31–134 and 31–150) used for human BTLA proteins?

    The different lengths of human BTLA proteins correspond to different isoforms. The aa31–134 sequence represents isoform 2, while aa31–150 corresponds to isoform 1. ACRO provides BTLA proteins of varying lengths to meet diverse customer requirements.

バックグランド

B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    B- and T-lymphocyte attenuator

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    6 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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