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製品詳細
Synonyms
BTLA, CD272
Source
Human BTLA (31-150), His Tag (BTA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.7 kDa. The protein migrates as 27-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human BTLA (31-150), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human BTLA (31-150), His Tag (Cat. No. BTA-H52H3) is more than 90% and the molecular weight of this protein is around 19-29 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human BTLA (31-150), His Tag (Cat. No. BTA-H52H3) at 5 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag (Cat. No. HVM-H5258) with a linear range of 0.005-0.313 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human BTLA (31-150), His Tag (Cat. No. BTA-H52H3) on NTA Biosensor, can Human HVEM, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 20.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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よくある質問
- Product
Why are different expression ranges (31–134 and 31–150) used for human BTLA proteins?
The different lengths of human BTLA proteins correspond to different isoforms. The aa31–134 sequence represents isoform 2, while aa31–150 corresponds to isoform 1. ACRO provides BTLA proteins of varying lengths to meet diverse customer requirements.
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最新の進展
医薬品開発の進捗- 英語名：
B- and T-lymphocyte attenuator
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
6 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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