Biotinylated Human BTLA (31-150) Protein, His,Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
BTA-H82E4-25ug
$360.00
BTA-H82E4-200ug
$1565.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    BTLA, CD272

  • Source

    Biotinylated Human BTLA (31-150), His,Avitag (BTA-H82E4) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).

    Request for sequence
  • Molecular Characterization

    BTLA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 28-34 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    BTLA SDS-PAGE

    Biotinylated Human BTLA (31-150), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    BTLA SEC-MALS

    The purity of Biotinylated Human BTLA (31-150), His,Avitag (Cat. No. BTA-H82E4) is more than 85% and the molecular weight of this protein is around 25-34 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     BTLA ELISA

    Immobilized Biotinylated Human BTLA (31-150), His,Avitag (Cat. No. BTA-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 2-78 ng/mL (QC tested).

    Protocol

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よくある質問

  • Product

    Why are different expression ranges (31–134 and 31–150) used for human BTLA proteins?

    The different lengths of human BTLA proteins correspond to different isoforms. The aa31–134 sequence represents isoform 2, while aa31–150 corresponds to isoform 1. ACRO provides BTLA proteins of varying lengths to meet diverse customer requirements.

バックグランド

B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    B- and T-lymphocyte attenuator

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    6 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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