Biotinylated Human BTLA (31-150) Protein, Fc,Avitag™ (MALS verified)

Customer Reviews
Cat. No. / Size
Price
Qty
BTA-H82F3-25ug
$360.00
BTA-H82F3-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    BTLA, CD272

  • Source

    Biotinylated Human BTLA (31-150), Fc,Avitag (BTA-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).

    Predicted N-terminus: Lys 31

    Request for sequence
  • Molecular Characterization

    BTLA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • SDS-PAGE

    BTLA SDS-PAGE

    Biotinylated Human BTLA (31-150), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    BTLA SEC-MALS

    The purity of Biotinylated Human BTLA (31-150), Fc,Avitag (Cat. No. BTA-H82F3) is more than 85% and the molecular weight of this protein is around 100-140 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     BTLA ELISA

    Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150), Fc,Avitag (Cat. No. BTA-H82F3) with a linear range of 0.02-0.625 μg/mL (QC tested).

    Protocol

Customer Reviews
Writing Reviews

FAQ

  • Product

    Why are different expression ranges (31–134 and 31–150) used for human BTLA proteins?

    The different lengths of human BTLA proteins correspond to different isoforms. The aa31–134 sequence represents isoform 2, while aa31–150 corresponds to isoform 1. ACRO provides BTLA proteins of varying lengths to meet diverse customer requirements.

Background

B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

Recent Advances

 
Drug Development Progress
  • English Name:

    B- and T-lymphocyte attenuator

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    6 Details

  • Highest Development Stage:

    Phase 3 Clinical

Contact Us
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • FAQ
  • Background
Contact Us

お問い合わせ
グローバル:+1 800-810-0816(Toll Free)

メールアドレス
order.jp@acrobiosystems.com

アクロバイオシステムズ株式会社
〒101-0051
東京都千代田区神田神保町1-32-8