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Product Details
Synonyms
ICE, IL1BC, P45
Source
Human Caspase-1 Protein, His Tag&Tag Free (CA1-H51W3) is expressed from E. coli cells. It contains AA Asn 120 - Asp 297 & Ala 317 - His 404 (Accession # P29466-1).
Predicted N-terminus: Met & Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.9 kDa & 10.4 kDa. The protein migrates as 9 kDa and 23-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.5 M Arginine, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Caspase-1 Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
Measured by its ability convert the substrate acetyl-Tyr-Val-Ala-Asp p-nitroanilide (Ac-YVAD-pNA) to p-nitroaniline (pNA). The specific activity is >50 pmol/min/µg (QC tested).
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