製品詳細
Synonyms
CCL1, I-309, C-C motif chemokine 1
Source
Rat CCL1 Protein, Fc Tag (CC1-R5253) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Cys 92 (Accession # D3ZDY4).
Predicted N-terminus: Lys 24
Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 34.4 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Rat CCL1 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Rat CCL1 Protein, Fc Tag (Cat. No. CC1-R5253) is more than 95% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human CCR8 Protein (VLP) (Cat. No. CC8-H52P5) at 5 μg/mL (100 μL/well) can bind Rat CCL1 Protein, Fc Tag (Cat. No. CC1-R5253) with a linear range of 0.02-2.5 μg/mL (QC tested).
Protocol
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