Human CCL14 / HCC-1 Protein, His Tag

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Cat. No. / Size
Price
Qty
CC4-H5249-100ug
$360.00
CC4-H5249-1mg
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    C-C motif chemokine 14, Chemokine CC-1, CC-3, HCC-1, HCC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, CCL14, NCC2, SCYA14

  • Source

    Human CCL14, His Tag (CC4-H5249) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Asn 93 (Accession # Q16627-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    CCL14 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 10.7 kDa. The protein migrates as 14-15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CCL14 SDS-PAGE

    Human CCL14, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

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Background

CCL14 has weak activity on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.

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