Human CD2 / SRBC Protein, Fc Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
CD2-H5258-50ug
$360.00
CD2-H5258-1mg (500ug X 2)
$2720.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD2, SRBC, LFA-2, T11

  • Source

    Human CD2, Fc Tag (CD2-H5258) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 209 (Accession # AAH33583).

    Predicted N-terminus: Lys 25

    Request for sequence
  • Molecular Characterization

    CD2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD2 SDS-PAGE

    Human CD2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD2 SEC-MALS

    The purity of Human CD2, Fc Tag (Cat. No. CD2-H5258) is more than 95% and the molecular weight of this protein is around 100-130 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD2 ELISA

    Immobilized Human CD58, His Tag (Cat. No. LF3-H5225) at 10 μg/mL (100 μL/well) can bind Human CD2, Fc Tag (Cat. No. CD2-H5258) with a linear range of 5-156 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     CD2 BLI

    Loaded Human CD2, Fc Tag (Cat. No. CD2-H5258) on Protein A Biosensor, can bind Human CD58, His Tag (Cat. No. LF3-H5225) with an affinity constant of 2.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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バックグランド

T-cell surface antigen CD2 is also known as Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5 and SRBC, is a single-pass type I membrane protein found on the surface of T cells and natural killer (NK) cells. CD2 is a member of the immunoglobulin superfamily. CD2 / SRBC contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD2 / SRBC interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3 / CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. In addition to its adhesive properties, CD2 also acts as a co-stimulatory molecule on T and NK cells. CD2 is a specific marker for T cells and NK cells, and can therefore be used in immunohistochemistry to identify the presence of such cells in tissue sections.

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医薬品開発の進捗
  • 英語名:

    T-cell surface antigen CD2

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    3 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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