PE-Labeled Human CD20 / MS4A1 Full Length Protein, His Tag (Nanodisc) (Site-specific conjugation)

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Cat. No. / Size
Price
Qty
CD0-HP2E4-25tests
$1470.00
CD0-HP2E4-200tests
$7320.00
ETA of in-stock products:2 business days

Product Details

  • Application

    1. Cell based assay (FACS or functional assay)
    2. Affinity testing (SPR / BLI)
    3. Screening (ELISA)
  • Synonyms

    MS4A1, CD20, MS4A-1

  • Source

    Nanodisc PE-Labeled Human CD20 Full Length Protein, His Tag (CD0-HP2E4) is produced via site-specific conjugation of PE to Human CD20 Full Length Protein, His Tag under optimal conditions with a proprietary technology. Human CD20 Full Length Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).

    Predicted N-terminus: Met 1

    Request for sequence

  • Molecular Characterization

    CD20 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.9 kDa.

    *The isotype control of empty/mock nanodisc (Cat. No. APO-HP1E3) is sold separately and not included in protein, you can follow the link for product information.

    1. Structure

      Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.

    2. Synthesis Process

      Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     CD20 FACS

    5e5 of Anti-CD20 CAR-293 cells were stained with 100 μL of 1:20 dilution (5 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD20 Full Length Protein, His Tag (Cat. No. CD0-HP2E4) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).​

    Protocol

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Background

A B-lymphocyte surface antigen expressed from pre-B to mature B cells, but absent on plasma cells. It functions as a calcium channel and is a validated immunotherapy target, famously exploited by rituximab for treating B-cell malignancies and autoimmune disorders.

Recent Advances

 
Drug Development Progress
  • English Name:

    B-lymphocyte antigen CD20

  • Category:

  • Approved Drugs:

    35 Details

  • Drugs in Clinical Trials:

    132 Details

  • Highest Development Stage:

    Approved

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