Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
CD7-M5252-100ug
$395.00
CD7-M5252-1mg (200ug X 5)
$2615.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD27, TNFRSF7, S152, T14, Tp55

  • Source

    Mouse CD27 Protein, Mouse IgG2a Fc Tag (CD7-M5252) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Arg 182 (Accession # P41272-1).

    Predicted N-terminus: Thr 21

    Request for sequence
  • Molecular Characterization

    CD27 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD27 SDS-PAGE

    Mouse CD27 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    CD27 SEC-MALS

    The purity of Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) is more than 95% and the molecular weight of this protein is around 95-125 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD27 ELISA

    Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL (QC tested).

    Protocol
  •  CD27 ELISA

    Immobilized Mouse CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand Protein, Fc Tag with a linear range of 1-16 ng/mL (Routinely tested).

    Protocol

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バックグランド

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    CD27 antigen

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    4 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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