FITC-Labeled Human CD38 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CD8-HF2H5-25ug
$330.00
CD8-HF2H5-200ug
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD38, T10, cADPr hydrolase 1

  • Source

    FITC-Labeled Human CD38, His Tag (CD8-HF2H5) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1). It is the FITC labeled form of Human CD38, His Tag (Cat. No. CD8-H5224).

    Predicted N-terminus: Val 43

    Request for sequence
  • Molecular Characterization

    CD38 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • Protein Ratio

    The FITC to protein molar ratio is 2-4.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD38 SDS-PAGE

    FITC-Labeled Human CD38, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    CD38 SEC-MALS

    The purity of FITC-Labeled Human CD38, His Tag (Cat. No. CD8-HF2H5) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD38 ELISA

    Immobilized Anti-CD38/Daratumumab, Human IgG1 (CH1/CH3 mutation) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD38, His Tag (Cat. No. CD8-HF2H5) with a linear range of 0.078-5 μg/mL (QC tested).

    Protocol
  • Bioactivity-FACS

     CD38 FACS

    2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human CD38 Protein, His Tag (MALS verified) (Cat. No. CD8-HF2H5) and negative control protein respectively. FITC signal was used to evaluate the binding activity (Routinely tested).

    Protocol

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Background

CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

Recent Advances

 
Drug Development Progress
  • English Name:

    Lymphocyte differentiation antigen CD38

  • Category:

  • Approved Drugs:

    3 Details

  • Drugs in Clinical Trials:

    47 Details

  • Highest Development Stage:

    Approved

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