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Product Details
Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
APC-Labeled Human CD3E & CD3D Protein, His Tag (CDD-HA2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 & Phe 22 - Ala 105 (Accession # P07766-1 & P04234-1).
Predicted N-terminus: Asp 23 & Phe 22
Molecular Characterization
The protein has a calculated MW of 29.9 kDa & 13.4 kDa.
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-FACS
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag Free (Cat. No. CDD-HA2H3) and negative control protein respectively, APC signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of APC-Labeled Human CD3E & CD3D Protein, His Tag&Tag Free (Cat. No. CDD-HA2H3) and negative control protein respectively, APC signal was used to evaluate the binding activity (QC tested).
Protocol
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