Cynomolgus / Rhesus macaque CD47 Protein, Fc Tag, low endotoxin

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製品番号/仕様
価格
数量
CD7-C5252-100ug
$360.00
CD7-C5252-1mg (250ug X 4)
$2245.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CD47, MER6, IAP, OA3

  • Source

    Cynomolgus / Rhesus macaque CD47, Fc Tag (CD7-C5252) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 141 (Accession # F7A802-1). In the region Gln 19 - Glu 141, the amino acid sequences of Cynomolgus and Rhesus macaque CD47 are identical.

    Predicted N-terminus: Gln 19

    Request for sequence
  • Molecular Characterization

    CD47 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition, and 100-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    CD47 SDS-PAGE

    Cynomolgus / Rhesus macaque CD47, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     CD47 ELISA

    Immobilized Mouse SIRP alpha, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 10 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, Fc Tag (Cat. No. CD7-C5252) with a linear range of 0.039-0.625 μg/mL (QC tested).

    Protocol

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バックグランド

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Cluster of differentiation 47

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    53 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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