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製品詳細
Synonyms
CD47, MER6, IAP, OA3
Source
Human CD47 Protein, Mouse IgG2a Fc Tag (CD7-H52A5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # Q08722-3).
Predicted N-terminus: Gln 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 40.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human CD47 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human SIRP alpha, His Tag (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Human CD47 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-H52A5) with a linear range of 1-31 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human CD47 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-H52A5) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Human CD47 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-H52A5) can bind to ACHN cell expressing human SIRP-a. The concentration of CD47 used is 10 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=8.39 μg/mL
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Cluster of differentiation 47
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
53 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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