製品詳細
Synonyms
CD8 alpha & beta, CD8A & CD8B
Source
Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (CDA-C52W3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # XP_065382910.1 & XP_005575417.1).
Predicted N-terminus: Ser 22 & Leu 22
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 23.7 kDa (CD8 alpha) and 21.1 kDa (CD8 beta). Subunit CD8 alpha is fused with with a polyhistidine tag at the C-terminus and subunit CD8 beta contains no tag. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-C52W3) is more than 85% and the molecular weight of this protein is around 45-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Cynomolgus CD8A&CD8B Heterodimer Protein, His Tag&Tag Free (Cat. No. CDA-C52W3) at 1 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
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最新の進展
- 英語名:
T-cell surface glycoprotein CD8 beta chain
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- 最新の開発ステージ:
Preclinical
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