Biotinylated Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Synonyms

CD8 alpha & beta, CD8A & CD8B

Source

Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (CDA-H82W7) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # P01732 & P10966-1).

Predicted N-terminus: Ser 22 & Leu 22

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 26.0 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit CD8 beta contains no tag at the C-terminus. The protein migrates as 33 kDa and 35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Endotoxin

Less than 0.1 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process