Cynomolgus DR3 / TNFRSF25 Protein, His Tag, low endotoxin (SPR verified)

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Cat. No. / Size
Price
Qty
DR3-C52H3-100ug
$355.00
DR3-C52H3-1mg
$2335.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1

  • Source

    Cynomolgus DR3 Protein, His Tag (DR3-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 20 - Gly 203 (Accession # G7NTB5-1).

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  • Molecular Characterization

    DR3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.7 kDa. The protein migrates as 33-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    DR3 SDS-PAGE

    Cynomolgus DR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-SPR

     DR3 SPR

    Cynomolgus DR3 Protein, His Tag (Cat. No. DR3-C52H3) immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque TL1A / TNFSF15, His Tag (Cat. No. TLA-C5241) with an affinity constant of 94.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.

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