TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (HPLC verified)

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Cat. No. / Size
Price
Qty
ENE-T52H4-50ug
$465.00
ENE-T52H4-500ug
$2885.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Envelope protein E/E protein (TBEV)

  • Source

    TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (ENE-T52H4) is expressed from human 293 cells (HEK293). It contains AA Ser 281-Lys 675 (Accession # P14336).

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.1 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Envelope protein E/E protein (TBEV) SDS-PAGE

    TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    Envelope protein E/E protein (TBEV) SEC-HPLC

    The purity of TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (Cat. No. ENE-T52H4) was greater than 95% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     Envelope protein E/E protein (TBEV) ELISA

    Immobilized TBEV (European subtype, strain Neudoerfl) Envelope protein E, His Tag (Cat. No. ENE-T52H4) at 1 μg/mL (100 μL/well) can bind Anti-Envelope protein E, Tick-borne encephalitis virus European subtype (strain Neudoerfl) antibody with a linear range of 0.001-0.125 μg/mL (QC tested).

    Protocol

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Background

Tick-borne encephalitis virus (TBEV), which belongs to Family Flaviviridae, is the leading cause of Tick-borne encephalitis in human and animals across Europe and East Asia. The infection of TBE virus will lead to the illness called Tick-borne encephalitis with high morbidity and mortality. TBE virus has three regional subtypes of European subtype (TBEV-Eu), Far-eastern subtype TBEV (TBEV-FE) and Siberian subtype (TBEV-Sib) that dominate their respective regions; however, the sequence variety at amino acid level is quite low and the European subtypes’ vaccines show cross protection against other subtypes. The envelope protein E is the major viral surface antigen that plays an important role in viral infection, making it a great target for vaccine development.

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