Human EphA2 Protein, His Tag, low endotoxin (MALS verified)

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製品番号/仕様
価格
数量
EP2-H52H4-100ug
$230.00
EP2-H52H4-1mg (500ug X 2)
$1650.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    EphA2

  • Source

    Human EphA2 Protein, His Tag (EP2-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 534 (Accession # P29317-1).

    Predicted N-terminus: Ala 24

    Request for sequence
  • Molecular Characterization

    EphA2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 58.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    EphA2 SDS-PAGE

    Human EphA2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    EphA2 SEC-MALS

    The purity of Human EphA2 Protein, His Tag (Cat. No. EP2-H52H4) is more than 95% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     EphA2 ELISA

    Immobilized Human EphA2 Protein, His Tag (Cat. No. EP2-H52H4) at 5 μg/mL (100 μL/well) can bind Human Ephrin-A1, Fc Tag (Cat. No. EF1-H5251) with a linear range of 0.06-1 ng/mL (QC tested).

    Protocol

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バックグランド

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Ephrin type-A receptor 2

  • カテゴリ:

  • 市販の医薬品数:

    2 詳細

  • 治験医薬品数:

    10 詳細

  • 最新の開発ステージ:

    Approved

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