Cynomolgus Fc epsilon RI alpha Protein, Fc Tag (BLI verified)

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製品番号/仕様
価格
数量
FCA-C5255-100ug
$310.00
FCA-C5255-1mg
$2245.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    FCER1A, FCE1A, FcERI

  • Source

    Cynomolgus Fc epsilon RI alpha, Fc Tag (FCA-C5255) is expressed from human 293 cells (HEK293). It contains AA Val 29 - Lys 204 (Accession # A0A7N9DA09-1).

    Predicted N-terminus: Val 29

    Request for sequence
  • Molecular Characterization

    Fc epsilon RI alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 46.9 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Fc epsilon RI alpha SDS-PAGE

    Cynomolgus Fc epsilon RI alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     Fc epsilon RI alpha ELISA

    Immobilized Cynomolgus Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-C5255) at 5 μg/mL (100 μL/well) can bind Cynomolgus IgE Fc Protein, His Tag (Cat. No. IGE-C52H3) with a linear range of 0.6-20 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-BLI

     Fc epsilon RI alpha BLI

    Loaded Cynomolgus Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-C5255) on AHC Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 1.65 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

    Protocol

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バックグランド

High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    IgE Fc receptor, alpha-subunit

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    1 詳細

  • 最新の開発ステージ:

    Approved

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