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Product Details
Synonyms
FCGR2B, C, CD32b, c, FcRII-b, c, Fc-gamma RII-b, c, Fc-gamma-RIIb, c, CD32, FCG2, IGFR2, CDw32
Source
Biotinylated Human CD32b/c, Avitag,His Tag (CDB-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1). In the region Ala 46 - Pro 217, the AA sequence of Fc gamma RIIB and Fc gamma RIIC are homologus.
Predicted N-terminus: Ala 46
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 23.0 kDa. The protein migrates as 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human CD32b/c, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) is more than 95% and the molecular weight of this protein is around 24-36 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 5 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Immobilized Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) on SA Chip, can bind Rituximab with an affinity constant of 4.22 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human CD32b/c, Avitag,His Tag (Cat. No. CDB-H82E0) on SA Biosensor, can bind Rituximab with an affinity constant of 2.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
Recent Advances
Drug Development Progress- English Name：
Low affinity immunoglobulin gamma Fc region receptor II-b
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Phase 3 Clinical
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