Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (MALS & SPR verified)

Synonyms

FcRn, FCGRT & B2M

Source

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-P5280) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M) (Accession # Q6XAV9-1 (FCGRT) & Q07717-1 (B2M)).

Predicted N-terminus: Asp 16 (FCGRT) & Val 21 (B2M)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 12.8 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 32-35 kDa (FCGRT) and 14 kDa (B2M) respectively.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process