Human FGF-10 / KGF 2 Protein, His Tag, low endotoxin

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Cat. No. / Size
Price
Qty
FG0-H5147-20ug
$165.00
FG0-H5147-50ug
$300.00
FG0-H5147-1mg (500ug X 2)
$2840.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    FGF-10, Fibroblast growth factor 10, Keratinocyte growth factor 2

  • Source

    Human FGF-10 Protein, His Tag (FG0-H5147) is expressed from E. coli cells. It contains AA Gln 38 - Ser 208 (Accession # O15520-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    FGF-10 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 21.3 kDa. The protein migrates as 25-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FGF-10 SDS-PAGE

    Human FGF-10 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     FGF-10 ELISA

    Immobilized Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 0.1-6 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     FGF-10 SPR

    Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) immobilized on CM5 Chip can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 4.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  • Application Data

     FGF-10 APPLICATION DATA

    Human FGF-10 Protein, His Tag (Cat. No. FG0-H5147) could support mouse gastric organoid culture after several passages, combined with Human EGF Protein, premium grade (Cat. No. EGF-H5117), Human Noggin Protein, Fc Tag, premium grade (Cat. No. NON-H5257), Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220).

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Background

Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.

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