Human FUS Protein, MBP,His Tag

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製品番号/仕様
価格
数量
FUS-H5144-100ug
$360.00
FUS-H5144-1mg (100ug X 10)
$2380.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fus-like protein, Oncogene FUS, Oncogene TLS, RNA-binding protein FUS, POMP75, Translocated in liposarcoma, Translocated in liposarcoma protein

  • Source

    Human FUS Protein, MBP,His Tag (FUS-H5144) is expressed from E. coli cells. It contains AA Met 1 - Tyr 526 (Accession # P35637-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    FUS Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a MBP tag at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 97.0 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    FUS SDS-PAGE

    Human FUS Protein, MBP,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     FUS ELISA

    Immobilized Human FUS Protein, MBP,His Tag (Cat. No. FUS-H5144) at 1 μg/mL (100 μL/well) can bind FUS antibody, Mouse IgG with a linear range of 0.2-4 ng/mL (QC tested).

    Protocol

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バックグランド

FUS (RNA-binding protein FUS) is a DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. It binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay.
FUS plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, FUS plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    RNA-binding protein FUS

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    2 詳細

  • 最新の開発ステージ:

    Phase 3 Clinical

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