Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0 Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
RSF-M82E3-200ug
$1520.00
RSF-M82E3-1mg (200ug X 5)
$5080.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Fusion glycoprotein F0

  • Source

    Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0 Protein, His,Avitag (RSF-M82E3) is expressed from human 293 cells (HEK293). It contains AA Val 20 - ILe 486 (Accession # P11236).

    Predicted N-terminus: Val 20

    Request for sequence
  • Molecular Characterization

    Fusion glycoprotein F0 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 53.6 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Fusion glycoprotein F0 SDS-PAGE

    Biotinylated Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

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Background

The two surface glycoproteins of the mumps virus are the hemagglutinin-neuraminidase (HN) and Fusion proteins. These glycoproteins are essential for viral entry to host cells, and the spread of newly formed virions. The mumps fusion protein (F) is a 538-amino acid, class one fusion surface glycoprotein. It is responsible for the membrane fusion of virus and host cell. The un-cleaved protein has three hydrophobic regions: an amino-terminal signal peptide, an amino terminal region of F1 and the carboxyl-terminal membrane domain. This protein starts as a precursor molecule (F0), and is then cleaved into the active protein by the recognition of a R-X-L/R-R motif by a host endoprotease (furin). The F protein contains two disulfide-linked polypeptides (F1 and F2).

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Drug Development Progress
  • English Name:

    Respiratory syncytial virus F protein

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  • Approved Drugs:

    3 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Approved

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