Biotinylated Nipah virus Fusion glycoprotein, His Tag, ultra sensitivity (primary amine labeling)

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製品番号/仕様
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FUN-N82H3-25ug
$399.00
FUN-N82H3-200ug
$1648.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    Fusion glycoprotein, F protein, F, Nipah virus, Hendra virus

  • Source

    Biotinylated Nipah virus Fusion glycoprotein, His Tag, primary amine labeling (FUN-N82H3) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Ser 487 (Accession # Q9IH63-1).

    Predicted N-terminus: Ile 27

    Request for sequence
  • Molecular Characterization

    Fusion glycoprotein Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.9 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Fusion glycoprotein SDS-PAGE

    Biotinylated Nipah virus Fusion glycoprotein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Fusion glycoprotein ELISA

    Immobilized Biotinylated Nipah virus Fusion glycoprotein, His Tag, primary amine labeling (Cat. No. FUN-N82H3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Fusion Protein Antibody, Human IgG1 (5B3) with a linear range of 0.06-2 ng/mL (QC tested).

    Protocol

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バックグランド

Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses discovered in the mid-to late 1990s that possess a broad host tropism and are known to cause severe and often fatal disease in both humans and animals. HeV and NiV infect host cells through the coordinated efforts of two envelope glycoproteins. The G glycoprotein attaches to cell receptors, triggering the fusion (F) glycoprotein to execute membrane fusion. G is a type II homotetrameric transmembrane protein responsible for binding to ephrinB2 or ephrinB3 (ephrinB2/B3) receptors. F is a homotrimeric type I transmembrane protein that is synthesized as a premature F0 precursor and cleaved by cathepsin L during endocytic recycling to yield the mature, disulfide-linked, F1 and F2 subunits. Upon binding to ephrinB2/B3, NiV G undergoes conformational changes leading to F triggering and insertion of the F hydrophobic fusion peptide into the target membrane. Subsequent refolding into the more stable post-fusion F conformation drives merger of the viral and host membranes to form a pore for genome delivery to the cell cytoplasm.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Respiratory syncytial virus F protein

  • カテゴリ:

  • 市販の医薬品数:

    3 詳細

  • 治験医薬品数:

    7 詳細

  • 最新の開発ステージ:

    Approved

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