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Product Details
Synonyms
GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
Source
Human GLP1R Protein, His Tag (GLR-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Glu 139 (Accession # P43220-1).
Predicted N-terminus: Ala 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.6 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human GLP1R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) is more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) at 2 μg/mL (100 μL/well) can bind Mouse Monoclonal GLP-1R Antibody with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-GLPR1 antibody captured on Protein A Chip can bind Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) with an affinity constant of 9.82 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) immobilized on CM5 Chip can bind GLP-1 (7-37) with an affinity constant of 1.07 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human GLP1R Protein, His Tag (Cat. No. GLR-H52H7) on NTA Biosensor, can bind GLP-1 (7-37) with an affinity constant of 1.80 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Glucagon-like peptide-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
13 Details
- Highest Development Stage：
Phase 3 Clinical
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