Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) Envelope Glycoprotein (GP) Protein, His Tag

Synonyms

Glycoprotein/GP (EBOV)

Source

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag (ZE5-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Asp 637 (Accession # AAQ55048.1).

Predicted N-terminus: Ile 33

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.2 kDa. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) when calibrated against Star Ribbon Pre-stained Protein Marker in SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>85% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

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2. Quality Advantages
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