お気に入りに追加
製品詳細
Synonyms
GPC3, OCI5, Glypican-3, GTR2-2, MXR7, DGSX, SDYS , SGB, SGBS, SGBS1
Source
Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (GP3-HA2H5) is produced via conjugation of AF488 to Human Glypican 3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Glypican 3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
Predicted N-terminus: Gln 25
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
お客様はこちらも閲覧
データ表示
Evaluation of CAR expression
FACS Analysis of Anti-Glypican 3 CAR Expression
5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HA2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 488-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HA2H5) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
ユーザーレビュー コメントを投稿
バックグランド
最新の進展
医薬品開発の進捗- 英語名：
Glypican-3
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
57 詳細
- 最新の開発ステージ：
Phase 2 Clinical
お問い合わせ
メールアドレス
order.jp@acrobiosystems.com
お問い合わせ
グローバル：+1 800-810-0816(Toll Free)
医薬品候補ライセンス
