Product Details
Synonyms
H-2Kb & B2M
Source
PE-Labeled Mouse H-2Kb&B2M Tetramer Protein (H2M-MP2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 305 (H-2Kb) & Ile 21 - Met 119 (B2M) (Accession # P01901 (H-2Kb) & P01887 (B2M)).
Predicted N-terminus: Gly 22 & Ile 21
Request for sequenceMolecular Characterization
PE-Labeled Mouse H-2Kb&B2M Tetramer Protein is assembled by biotinylated monomer (H2M-M82E7) and PE-labeled streptavidin.
Biotinylated Mouse H-2Kb&B2M Monomer Protein is produced by co-expression of H-2Kb and B2M. This Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA

Immobilized PE-Labeled Mouse H-2Kb&B2M Tetramer Protein (Cat. No. H2M-MP2H7) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized PE-Labeled Mouse H-2Kb&B2M Tetramer Protein (Cat. No. H2M-MP2H7) at 1 μg/mL (100 μL/well) can bind Biotinylated anti-H-2Kb/H2D monoclonal antibody with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
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