Product Details
Synonyms
HA, Hemagglutinin
Source
Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag (HAB-V52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 16 - Thr 545 (Accession # EPI_ISL_20373028), GISAID).
Predicted N-terminus: Asp 16
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.8 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H4) is more than 90% and the molecular weight of this protein is around 205-250 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H4) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Influenza B/Victoria Lineage HA antibody-21E11, Human IgG1 | Human Kappa with a linear range of 0.01-1 ng/mL (QC tested).
Protocol
Immobilized Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H4) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Influenza B Virus HA antibody-16H6, Human IgG1 | Human Kappa with a linear range of 0.01-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Mouse Anti-Influenza B Virus HA antibody-13H8,Human IgG1 | Human Kappa immobilized on CM4 Chip can bind Influenza B virus (Tokyo/EIS13-175/2025) HA (B/Victoria lineage) Protein, His Tag (Cat. No. HAB-V52H4) with an affinity constant of 16.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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